Cryotop and development of vitrified immature bovine oocytes
نویسندگان
چکیده
منابع مشابه
Cryotop and development of vitrified immature bovine oocytes
The effectiveness of different cryodevices (open-pulled straw (OPS), electron microscopy grid (EMG), and Cryotop was evaluated for vitrification of immature bovine oocytes. Polar body, metaphase II stage (MII), survivability, and subsequent developmental rates were determined. Only oocytes with four or five layers of cumulus cells were used. Oocytes were equilibrated in two vitrification soluti...
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In vitro maturation of vitrified immature germinal vesicle (GV) oocytes is a promising fertility preservation option. We analyzed the ultrastructure of human GV oocytes after Cryotop vitrification (GVv) and compared it with fresh GV (GVc), fresh mature metaphase II (MIIc) and Cryotop-vitrified mature (MIIv) oocytes. By phase contrast microscopy and light microscopy, the oolemmal and cytoplasmic...
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In this work we analyze the effects of vitrification on the ultrastructure and developmental ability of bovine oocytes matured in the presence of 9-cis-retinoic acid (RA). Bovine cumulus oocyte complexes (COCs) were matured in a basic medium containing 10% fetal calf serum (FCS), 9-cis-RA or polyvinyl alcohol (PVA). Mature oocytes were vitrified using the Open Pulled Straw method with minor mod...
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Purpose: This Study evaluated the resumption of meiosis, in vitro maturtion and department of immature mouse oocytes with b- mercaptothanol. Materials and methods: Immature oocytes were recovered from NMRI mouse strain (4-6 weeks) in different 4 experiments groups: the control group, the oocytes were matured in MEM-a+ medium containing + FCS 55%; group 1: the oocytes were matured in MEM- a med...
متن کاملComparison of Cryotop and micro volume air cooling methods for cryopreservation of bovine matured oocytes and blastocysts
This study was designed to compare the efficiency of the Cryotop method and that of two methods that employ a micro volume air cooling (MVAC) device by analyzing the survival and development of bovine oocytes and blastocysts vitrified using each method. In experiment I, in vitro-matured (IVM) oocytes were vitrified using an MVAC device without direct contact with liquid nitrogen (LN2; MVAC grou...
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ژورنال
عنوان ژورنال: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
سال: 2011
ISSN: 0102-0935
DOI: 10.1590/s0102-09352011000100011